The method enabled detection of 100 pg of standard GW1126 strain or 102 copies of pGW1126 plasmid with the concentration determined as 1.32 μg/μL and was considered as very sensitive (Figure 2).
Raue R, Schmidt V, Freick M, Reinhardt B, Johne R, Kamphausen L, Kaleta EF, Müller H, Krautwald-Junghanns ME: A comprehensive study on a disease complex associated with pigeon circovirus infection, young pigeon disease syndrome. 10.1080/03079450500267825. The second group was represented by a single human herpesvirus 1 whilst the third less homogeneous group was composed of sequences of poultry herpesviruses. Columbid alphaherpesvirus 1 strain HLJ, complete genome. J Virol 71:2072–2082, CAS Propagated on the CAM it produces plaques and kills the embryo in 4 days. Therefore, we considered them specific only for the DNA-dependant DNA polymerase gene of CoHV-1.
Google Scholar, Duchatel JP, Vindervogel H (2008) Miscellaneous herpesvirus infections. The virus is antigenically related to CoHV-1 and was found in falcons and owls [1, 4, 5, 12–16]. In July 2012, increased mortality was observed in a hybrid meat-type pigeon flock in Beijing, China. Virus Gene 36:269–278, CAS Correspondence to Columbid herpesvirus 1 CoHV1 An. Sileo L, Carlson C, Crumley S: Inclusion body disease in a great horned owl. SP provided infectious materials from falcons with detailed description and documentation. (A) Amplification in samples containing successive 10-fold dilutions of CoHV-1 standard isolate.
The falcons and owls are thought to have become infected during the ingestion of pigeon meat thus suggesting the virus’s capacity to infect a wide range of hosts. Transmission electron microscopy demonstrated viral particles typical of herpesvirus within hepatocyte nuclei and budding from the nuclear membrane. Clinical signs observed in young female of Peregrine Falcon (Falco peregrinus)showing torticollis and keratitis. Columbid herpesvirus 1 has been shown to infect not only pigeons but owls, falcons, and hawks as well. The cells were harvested 96 h p.i. Vet Rec 81:267–268, Das S, Noe JC, Paik S, Kitten T (2005) An improved arbitrary primed PCR method for rapid characterization of transposon insertion sites. However, it has been further reclassified as an Alphaherpesvirus because of its close relation to the Marek’s disease virus in chickens (MDV) [9, 10]. The study on CoHV-1 phylogenetic analysis among different free-ranging birds may provide additional information and evidence on the ubiquitous features of this virus. Wellehan, April J. Johnson, April L. Childress, Scott D. Fitzgerald, Michael J. Kinsel, Research output: Contribution to journal › Article › peer-review. Avian Pathol. Transmission electron microscopy demonstrated viral particles typical of herpesvirus within hepatocyte nuclei and budding from the nuclear membrane. The complete investigation should include full necropsy and histological assay which we were unable to conduct. Causes conjunctivitis, respiratory lesions and focal necrosis of the liver, but is usually carried by apparently normal birds as a latent infection. Cookies policy. The uniplex-PCR technique facilitating the detection of all three most important viral pathogens of pigeon was further described by Frieck et al.. Columbid herpesvirus-1 (CoHV-1) is widespread in feral pigeons ( Columba livia ) in North America and Europe. Meanwhile, there are many asymptomatic infections in pigeons older than 6 months. However, in the past two variants of neuropathogenic CoHV-1 strains occurred in rock pigeons from Iraq and Poland [22, 26]. Rose N, Warren AL, Whiteside D, Bidulka J, Robinson JH, Illanes O, Brookfield C: Columbid herpesvirus-1 mortality in great horned owls (Bubo virginianus) from Calgary, Alberta. In one case, necrosis and inclusions were also noted in bone marrow, thymus, bursa of Fabricius, thyroid gland, parathyroid gland, ceca, and the enteric system. Characterization of the first columbid herpesvirus 1 isolate from a hybrid meat-type pigeon flock in China. J Microbiol Methods 63:89–94, CAS ESS assisted in experimental design of the study. Histologic lesions included splenic and hepatic necrosis associated with eosinophilic intranuclear inclusion bodies characteristic of herpesvirus. © 2020 Springer Nature Switzerland AG.
The detection limit was determined as the highest dilution that resulted in a fluorescent signal plotted as a curve detected as cycle threshold (CT) value. Vet Rec. Die Infektion wird verursacht durch Viren der Spezies Tauben-Herpesvirus 1 (wissenschaftlich Columbid alphaherpesvirus 1, CoHV-1) in der Gattung Mardivirus der Unterfamilie Alphaherpesvirinae.
The results obtained in our study confirm previous findings that all recently reported herpesviruses from birds of prey belong to CoHV-1 [1, 5, 13, 14, 27]. However, virus isolation in cell cultures confirmed the CoHV-1 presence. Mozos E, Hervas J, Moyano T, Diaz J, Gomez-Villamandos J: Inclusion body disease in a peregrine falcon (Falco peregrinus): Histological and ultrastructural study. Complete genome sequence and evolution analysis of a columbid herpesvirus type 1 from feral pigeon in China. 10.2307/1590297. title = "Columbid herpesvirus-1 in two Cooper's hawks (Accipiter cooperii) with fatal inclusion body disease".
In one case, necrosis and inclusions were also noted in bone marrow, thymus, bursa of Fabricius, thyroid gland, parathyroid gland, ceca, and the enteric system. PCR results provide evidence that this disease is transmitted to raptors via Rock Pigeons, most likely through ingestion of Rock Pigeons as prey.". Vindevogel H et al (1985) J Comp Pathol 95, 105, Colonic carcinoma cell line CaCo2 See CACO2 cell line, Walleye herpesvirus See percid herpesvirus, Anterior poliomyelitis virus Synonym for Poliovirus. A similar identity (99.1-100%) was found by comparing examined CoHV-1 to two other foreign sequences originating from Germany and Australia. Not transmissible to chickens, ducks, geese or quail, but budgerigars and turtle doves can be infected experimentally. On the basis of the multiple alignment, analysis and neighbour-joining (NJ) computational method, the phylogenetic tree was constructed (Figure 4). Eighteen samples (20.4%) were positive for CoHV-1 (Table 1). Gunther BM, Klup BG, Gravendyck M, Lohr JE, Mettenleiter TC, Kaleta EF: Comparison of the genomes of 15 avian herpesvirus isolates by restriction endonuclease analysis. Diagnostic tests led to the isolation of a virus designated columbid herpesvirus 1 BJ strain (CoHV-1BJ).
The highest cycle threshold (CT) values – 17.1 and 21.7 – were found in rock pigeons from the city of Lublin (GJW1130 and GJW1126). Article
Meanwhile, MDV officially named as Gallid herpesvirus 2 (MDV-1), shares common genetic properties with other herpesviruses isolated in poultry including the infectious laryngotracheitis virus - ILTV (Gallid herpesvirus 1), MDV serotype 2 – MDV-2 (Gallid herpesvirus 3), and the previously mentioned PsHV-1 infecting parrots. 2011, 89: 193-196. The authors would like to thank Dr. Yanxin Hu at the College of Veterinary Medicine of China Agricultural University for the assistance provided with the histological slide preparation. Columbid herpesvirus 1 (CoHV-1) is a member of Herpesviridae family, a large family of DNA viruses that cause diseases in a host of animals including humans. Finally, the analysed CoHV-1 strains shared an identity from 32.7 to 36.3% with the related human herpesvirus 1 (Figure 3). This similarity was independent from host species. 10.1080/03079459708419213. The analytic method selection might explain this variation between the percentages of CoHV-1 positive birds since PCR-based techniques enable a more sensitive detection of viral pathogens in comparison to serological assays. Only one serotype has been found. 10.1080/03079459008418673. Columbid herpesvirus 1. qPCR as an approach to detecting and simultaneously quantifying pathogens is the method of choice given its advantages in speed, sensitivity and clear cut answers/scoring when compared with other classical methods.
What is worth mentioning, the samples of brain are not commonly used for CoHV-1 isolation and detection. The sensitivity of the real-time PCR was examined using 5 ten-fold dilutions (100 ng – 10 pg) of the entire GW1126 strain (200 ng/μL) as well as 5 ten-fold dilutions of pGJW1126 plasmid (from 108 to 101 copies). Our work was supported by the National Natural Science Foundation of China (No. 1945, 81: 385-398. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. All strains were passaged three times then submitted to DNA extraction and real-time PCR.
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